Perfecting An Artificial Pancreas
[ Follow-Ups ]
[ Post Followup ]
[ The Islet Foundation Public Message Forum ]
Posted by mariner on 08:41:38 2008/05/05
A number of research groups around the world are currently working to develop artificial pancreas technologies. Most strategies involve transplanting healthy islets the pancreatic cells that detect glucose and release insulin into the diabetic patient. The cells are encapsulated either individually within a hydrogel or as a large collection of cells within some type of polymer membrane.
Kennedy's group takes the latter approach. Their prototype begins with a scaffold made from a 7-cm-long tube of the biocompatible nickel-titanium alloy nitinol that's been perforated with laser-cut hexagonal holes. Polyurethane nanofibers are then electrospun over the tube, through techniques designed by Kennedy's colleague Miko Cakmak, who is a polymer engineering professor at Akron. The resulting entanglement of nanofibers, or so-called nanomats, reinforces the openings in the scaffold's mesh.
THE TUBE is then coated with a semipermeable polymer membrane made up of poly(dimethylacrylamide) and polydimethylsiloxane domains cross-linked by polymethylhydrosiloxane. The entire assembly is dried, washed to remove small molecules, cleaned in an autoclave, and finally filled with a suspension of pig islets.
Ken S. Rosenthal, a professor of microbiology and immunology at Northeastern Ohio Universities College of Medicine, and one of Kennedy's collaborators on the project, says that the polymer membrane is really what sets this artificial pancreas apart. Encapsulating a large collection of islets has been difficult, he says, because the material to make that capsule has never been designed for that purpose.
"This device differs because its polymer membrane has been designed to have the optimal properties for encapsulating islets," Rosenthal tells C&EN. "It allows for free movement of insulin and glucose but restricts access of immune molecules that might attack the encapsulated islets." Likewise, any viruses that might be piggybacking on the islets are trapped behind the membrane.
"Because of that, we can use pig cells, and the only thing that communicates between them and the patient are the small molecules and small proteins," Rosenthal notes.
The polymer can also sequester oxygen from the environment, thanks to its silicone-based components. This oxygen nourishes the encapsulated islets cells. "These membranes are biocompatible, flexible, transparent, autoclavable, and they're easily synthesized and relatively inexpensive," Rosenthal says.
"Kennedy is the only researcher that I know of who designed the bioartificial pancreas for longevity and oxygen permeability," says Len Pinchuk, president and chief executive officer of Miami-based Innovia, a company that develops biomaterials and medical devices. "Most other researchers in this field come from a biological background and don't have the tools to create these new biomaterials."
The artificial pancreas can be inserted anywhere in the body that encounters blood flow, Kennedy says, even just beneath the skin. "The blood brings the oxygen and nutrients needed by the islets and removes metabolic wastes, such as carbon dioxide."
The device, Kennedy notes, acts as both a glucose sensor and an insulin delivery device. "It delivers exactly the needed amount of insulin," he says. "Insulin concentration in the body must be precisely controlled. Too high or too low concentrations result in very serious side effects."
Follow Ups:
Post a Followup
[ Follow Ups ]
[ Post Followup ]
[ The Islet Foundation Public Message Forum ]